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Wasps dominated the first half, to go ahead 20-8 at halftime. Tries from Worsley and Van Gisbergen countered a score from Gloucester centre Anthony Allen that had taken Gloucester ahead in the 9th minute.
Gloucester then staged a dramatic comeback to surge ahead at the start of the second half, three tries in 20 minutes allowing them to take a 32-20 lead at the hour mark.
Wasps showed spirit and courage however, and conjured two tries from the last ten minutes, the first for Sackey and then a second in the match for Worlsey, to overhaul their hosts and run out the victors.
The win secures Wasps a semi-final tie with Sale at Edgeley Park next Sunday, kick off at 1.30pm.
Wasps have been allocated 500 tickets for supporters wishing to travel to the game next weekend - the same as for all Guinness Premiership matches. For those wishing to go to the game, Wasps are also laying on FREE coaches to get you to there.
Tickets will go on sale via the Ticket Hotline only. Season Ticket Holders will enjoy priority booking from 9.30am on Monday until 12 noon on Tuesday, after which any remaining tickets will go on general sale.
Supporters can also book a place on the FREE supporters buses at the same time as buying tickets. Buses will leave from London or High Wycombe, please state your preference of which bus you would like to travel on when booking you r tickets.
To book your tickets, and a place on Wasps FREE supporters buses, call the Ticket Hotline on 0870 414 1515.